Positive growth (i.e. The Simons reagent kit consists of two reagents in two separate bottles, marked Sa and Sb. In a unregulated market there is no way to ensure what youre buying is actually the intended drug, but with the use of test kits you can test your drugs and see what it actually is.With the growing trend of research chemicals being falsely sold, testing your drugs has never been more important. Identification of Drugs of Abuse, NIJ Standard 0604.01, National Uses. Hardy Diagnostics-SIMMONS CITRATE AGAR (L80). 0000014761 00000 n Part of Springer Nature. Simons is especially useful for differentiating between MDMA and MDA, as well as methamphetamine and amphetamine. It reacts with secondary amines like MDMA and methamphetamine to give a blue solution. Simon's reagent (a mixture of sodium nitroprusside, sodium carbonate, and acetaldehyde) is a chromogenic test used for the detection of alkaloids (containing secondary amines). Free Lighting Fast Shipping (3-6 days typically)~ When exposed to an amine, reaction . Intuitive Systems, Inc.- Simmons Citrate Agar Slant. To detect adulterants, try using on fresh samples some of the primary reagents like Marquis and Mecke, and secondary reagent tests likeRobadope, Scott,orZimmermann. In the early 1920s, Koser developed a liquid medium formulation for the differentiation of fecal coliforms from the coliform group. Szaserw 38/12,Warsaw The shift in pH turns the bromthymol blue indicator in the medium fromgreen to blue above pH 7.6. The reactions of this medium alone are not sufcient for identication to the species level. Sodium Chloride maintains the osmotic balance of the medium. The presence of MDMA/MDE/Methamphetamine/methylone will be indicated by a change to a bright cobalt blue. If your sample consists of MDMA or MDE, it will turn a blue color. 53 (1970) 1126. A. Oberlin, G. Terriere and J. L. Boulmier, Tanso (Japan) No. The Simon's reagent reacts preferentially with the more graphitic structures of the G-component unlike the Ts-component which appears to be structurally homogeneous. PMA is much more frequently seen in Europe, even in the last decade. Ofcourse! It also acts as a good negative control for amphetamines, since it should not react with them. NInfo.script= "load valium.mol; background white;"; Find company research, competitor information, contact details & financial data for Uwe Heller of Brhl, Baden-Wrttemberg. Soc. Simmons later modified this formulation to produce a solid medium that eliminated potential errors when interpreting growth. One litre of Benedict's reagent can be prepared by mixing 17.3 grams of copper sulfate pentahydrate (CuSO 4 .5H 2 O), 100 grams of sodium carbonate (Na 2 CO 3 ), and 173 grams of sodium citrate in distilled water (required quantity). ), however one other important drug we didnt mention is MDA (also known as sass or sassafras). H. Marsh and R. R. Adair, Carbon Colour Test Reagents/Kits for Preliminary Kovar, This website uses cookies to adjust and correctly display content. Spectrosc. Instructions, safe handling, storage and most importantly reactions charts. For tubes, dispense 4.0 to 5.0 ml into 16-mm tubes. So, if marquis turned orange, and mecke doesnt change color at all, you have another confirmation that you have some type of amphetamine instead of MDMA. / Telegram / Signal / WhatsApp: Simons should indeed not react to MDA, so that discoloration will be because of impurities. Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. 0000003358 00000 n Institute of Justice. Dancesafe. Out of these cookies, the cookies that are categorized as necessary are stored on your browser as they are essential for the working of basic functionalities of the website. I. Tomizuka and O. Watanabe, Yogyo Kyokai Shi (J. Ceram. A violet color indicates presence of ephedrine or pseudoephedrine, This page was last edited on 28 February 2023, at 18:34. Lower your dose with a DanceSafe branded "Less is More" pill cutter. Your email address will not be published. 0000007974 00000 n Using the test tube place one drop of RobaTest reagent and then 2 of buffer and swirl gently (to mix) and wait 30 to 60 seconds and note the colour. Links checked and/or last %%EOF All Rights Reserved. As this precursor became harder to find, its possible that unscrupulous precursor vendors sold fennel oil, which smells similar to safrole oil, to black-market labs looking to synthesize MDMA. Here, the copper (II) sulfate acts as a source of Cu 2+ ions, the sodium . Edit: simons reagent is sodium nitroprusside 1 g in 50 ml distilled water; add 2 ml acetaldehyde So this is completely different than marquis reagent, which is 100 ml concentrated sulfuric acid added to 5 ml 40% formaldehyde. Preparation of Benedict's Reagent. The reaction is used in spot tests and is also known as Chen-Kao test (or simply as Chen, Test T in UN Precursor Test Kit). Created October 2011. jmolApplet = Jmol.getApplet("jmolApplet0", NInfo); Oxidation with Simon's reagent and HNO3/H2SO4 of turbostratic graphitic carbon prepared catalytically by nickel particles (20nm) in a carbonized resin. Just head here and enter your results to find out what you got. HiMedia Laboratories Pvt. 0000006646 00000 n The inoculum should be from a pure, overnightculture grown on a solid medium and not froma broth suspension. Amphetamines(such as meth), which are also unfortunately sold as MDMA, will turn an orangish color. 0000005043 00000 n 109 (1977) 145. 0000159563 00000 n All orders ship same day if placed before 12PM CST. Rapid Mandelin is particularly useful for testing molly samples, because its able to detect a potential deadly adulterant that is now rarely(thankfully) sold as MDMA. If citrate can be used, the microbe will accumulate alkaline/basic byproducts. G. R. Millward, D. A. Jefferson and J. M. Thomas, ibid. If youre new to the drug testing scene, you likely have no idea what reagents you need, and may only have a vague idea of what they do. 0000002800 00000 n Delivery is 2-5 days depending on location. startxref 0 Comments Pay $10 each when you buy 30 or more. Replace the cap. 0000002305 00000 n If so, register here for discount pricing. I already recommended to friends. Well, we have already covered most of the common drugs that are sold as molly: MDMA, amphetamine, and bath salts(methylone/butylone etc. These labs then unknowingly synthesize PMA/PMMA, and the rest is history. Crystallogr. Simon's reagent was prepared as a solution of 5g silver dichromate in 25cm 3 concentrated sulphuric acid. Simons is what we call a binary reagent, meaning its basically a yes or no, and it only changes one color when its positive( a deep blue). The uninoculated medium will be a deep forest green due to the pH of the sample and the bromothymol blue. First and foremost they care about your general safety and take that into consideration. 0000125387 00000 n 0000010599 00000 n The results of the solvent extractions, summarized in Table II appear to add little novelty to the results of the original Chen-Kao reactions. Phone: (402) 235-9889 Email: info(at)wimscilabs.com Cool to approximately 25 C and dissolve 10 g of Na 2 S 2 O 5. So you cant always depend on the reagents to produce a specific reaction. However, it is known from previously published cross-testing work that various pharmaceuticals not related to the ephedrine group may produce similar blue copper complexes. 0000008748 00000 n The procedure is as follows: Place small amount of material to be tested on a spot plate. Shipped discretely. February 1989. //]]> In order to further differentiate between ephedrine enantiomers - ephedrine, pseudoephedrine and other ephedrine-related compounds, Simons test (with acetaldehyde) and Simons test (with acetone) are used. It's best to use Simon's reagent to test PMMA vs PMA, MDMA vs MDA, or meth/amphetamine, but it's also a good test for mephedrone and other secondary amines. Simon's Reagent Testing Kit $ 20.00 Simon's is a secondary test that is almost always used alongside at least one other reagent. D. Crawford and H. Marsh, J. Microscopy Also, the violet color obtained in the reaction is easy to define. NOTE: Simons can only test the PRESENCE, not PURITY or QUANTITY of a specific substance. M. Oberlin and J. Mering, Carbon Subscribe for new videos - Thanks for keeping it real.Reagent Kit Used: http://bunkpolice.com/simons-reagent-kit/USING THIS VIDEO FOR OTHER TEST KITS MAY PRO. //]]>. 0000005402 00000 n For MDMA and Marquis reagent, the color change thats seen (when its pure) is a deep purple to near black color. Electron Once you have that baseline colour (~light pink) then add the substance to be tested. Squeeze TWO(2) DROPS of Sb out of the bottle onto the same powder/liquid already on the plate from step 2. Chemical Safety sp. doi:10.1371/journal.pone.0069609. World Customs Organisation (WCO) Illicit Trade Reports, Cobalt //84b*u{L (e =Jj8ruS; $" Overnight shipping available. This category only includes cookies that ensures basic functionalities and security features of the website. Simons is a secondary test that is almost always used alongside at least one other reagent. Find the best reagent test kits HERE. Chemicals, United Nations Scientific and Technical Notes Tests with equivocal results should be repeated. It should change immediately. Deionized water =1,000 mlFinal pH 6.9 +/- 0.2 at 25 degrees C. Simmons Citrate agar is used to test an organismsability to utilize citrate as a source of energy. The test is often used to distinguish ephedrine, pseudoephedrine, norephedrine, cathinone and methcathinone from amphetamine and methamphetamine, 0000125889 00000 n Bacteria that can grow on this medium producean enzyme, citrate-permease, capable of convertingcitrate to pyruvate. Molly Testing Kits: What Reagents Do I Need and Why. no citrate utilisation) the color of the medium remains unchanged.Examples:Escherichia coli,Shigella, Yersinia, Edwardsiella species, etc. and those who inject drugs. So every drug that Simons reacts with will theoretically have a secondary amine group. They must be used together for the . 002 and L Simmons Citrate Agar may be used to differentiate citrate-positive, Simmons Citrate Agar is primarily used to aidin the identification of Enterobacteriaceae. We have already described in another post, the chemistry behind why exactly marquis reagent works, but in short, it reacts with nitrogen groups in a compound to produce a specific color for each drug. 0000016266 00000 n 0000020961 00000 n A. Oberlin and J. P. Rauchy, Compt. 268 (1969) 660-c. A. Oberlin, M. Oberlin and J. R. Comte Trotet, J. Microsc. The results were dead on and it answered all my questions! Scroll or click "accept" to accept and continue. Simon's reagent is used as a simple spot-test to presumptively identify alkaloids as well as other compounds. Repeat with bottle B. Save my name and email in this browser for the next time I comment. G. Norwitz and M. Galan, ibid. 0000002179 00000 n "Die gegrillte Leber ist super" The execution of the Chen-Kao reaction is simple, needs little practice and limited skills. Issue 12, pages 1292-1299, December 1930. be the Marquis reagent is used as a simple spot-test to presumptively identify alkaloids as well as other compounds. What makes testing MDA tricky, is the fact that it has the same exact reactions as MDMA for marquis, mecke, AND mandelin. Thermo Fisher Scientific Inc.,Dehydrated Culture Media-SIMMONS CITRATE AGAR. Simon's Reagent: Solution A: Dissolve 1 g of sodium nitroprusside in 50 mL of distilled water and add 2 mL of acetaldehyde to the solution with thorough mixing. Subscribe and get 10% off your first order. Of all ephedrine-related compounds, only ephedrine and pseudoephedrine produce the typical, stable violet color. v.89-51669, Germany. The Simons Reagent kit is a secondary test (after the use of Marquis, Mecke, & Mandelin) that tells the difference between MDMA vs MDA, methamphetamine vs amphetamine, or between MDPV vs methylone. Every reagent order comes with a full explanatory pamphlet. <<41913147f5e7104eaf4d28b86439ae3e>]>> Preparation of Reagent: Used for: Chen- Kao Reagent: Reagent 1: This is prepared by adding 1 ml of Glacial Acetic Acid in 100 ml of Water, i.e., 1% . [CDATA[ Magnesium Sulfate is a cofactor for a variety of metabolic reactions. It is used for the differentiation of Gram-negative bacteria on the basis of citrate utilization. However, a slow transition of the initial color into yellow, followed by an orange-brown color can be observed with both compounds, thus indicating the instability of the complexes initially formed, and an obvious decomposition of the two compounds under the alkaline conditions of this color reaction. Always test under a strong light and using the smallest possible sample. Dipotassium Phosphate actsas a buffer. Japanese Journal of Toxicology and Environmental Health MASATOSHI SYOYAMA 1 (1964) 471. Shake for 3 minutes and incubate in the dark at room temperature for 24 hours. M. Nakamizo, M. Inagaki and Y. Hishiyama, Proceedings of the 13th Conference on Carbon, 1722 July (Irvine, California, 1977) p. 274. Great price too. You also have the option to opt-out of these cookies. 0000047097 00000 n Uses. Chem. To estimate purity of a substance (amount of adulterants and potency) use. Use just enough powder to see on the plate. Learn more about Institutional subscriptions. One year is to be expected in optimal storage conditions but shelf life varies with each reagent as well as to what conditions you have stored them. Scrape a tiny bit of your pill or powder onto a large, white ceramic plate. In both cases the surface of the medium is lightly inoculated by streaking and, where slopes are used, the butt of medium is inoculated by stabbing. So to put it simply, all our orders are packaged extremely discretely and with care of privacy. The X-ray diffraction patterns show that graphitization increased A
. Ammonium Dihydrogen Phosphate is the sole source of nitrogen. This is because structurally, MDMA and MDA and very similar. 0000002020 00000 n This is a preview of subscription content, access via your institution. Contains over 200 combined individual tests! |. by elevation | Jun 15, 2021 | Educational posts | 0 comments. Place small amount of material to be tested on a spot plate. Methamphetamine, Simon's and Robadope's Reagent, PLoS ONE 8(7): e69609. This doesnt just mean selling them cheaper than everyone else, though. Due to the nature of Robatest you need to add a little more than for most of the other reagents (Liebermann . DoseTest is here to shake up the harm reduction world, taking lifesaving materials like reagent test kits and fentanyl test strips from the small niche where they have lived and making them accessible to everyone. volume16,pages 18091814 (1981)Cite this article. Mail Us: WIM SCIENTIFIC LABORATORIES 3157 Farnam Street Suite 7104PMB7149 Omaha, NE 68131. Simon's A/B Reagent - WIM Scientific Laboratories Unlike other Simons A/B Reagent Providers we provide you with everything you need to identify illicit substances and compounds. Your email address will not be published. 13 (1975) 353. 1. And while PMA is rare in the US, was reported to be seen as recently as the year 2001 in a Mitsubishi pill sold as MDMA. 5 (1967) 287. Tollens' reagent(chemical formula Ag(NH3)2OH{\displaystyle {\ce {Ag(NH3)2OH}}}) is a chemical reagentused to distinguish between aldehydesand ketonesalong with some alpha-hydroxy ketoneswhich can tautomerize into aldehydes. 0000125457 00000 n 0000048082 00000 n 0000001811 00000 n Starting from the basics, reagent testing kits are chemical mixtures that react with a given compound(drug) such as MDMA, and produce a predictable color when they come in contact with that drug. H. Marsh and D. W. Taylor, unpublished results. It is one of three reagents in the MDMA kit. //